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| Acceso al texto completo restringido a Biblioteca INIA Las Brujas. Por información adicional contacte bibliolb@inia.org.uy. |
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Biblioteca (s) : |
INIA Las Brujas. |
Fecha : |
14/06/2022 |
Actualizado : |
14/06/2022 |
Tipo de producción científica : |
Abstracts/Resúmenes |
Autor : |
GARCÍA, A.; AGUILAR, I.; LEGARRA, A.; MILLER, S.; TSURUTA, S.; MISZTAL, I.; LOURENCO, D. |
Afiliación : |
ANDRÉ GARCÍA, University of Georgia; IGNACIO AGUILAR GARCIA, INIA (Instituto Nacional de Investigación Agropecuaria), Uruguay; ANDRÉS LEGARRA, UMR GenPhySE, INRA; STEPHEN P. MILLER, Angus Genetics Inc; SHOGO TSURUTA, University of Georgia; IGNACY MISZTAL, University of Georgia; DANIELA LOURENCO, University of Georgia. |
Título : |
Accuracy of indirect predictions for large datasets based on prediction error covariance of SNP effects from single-step GBLUP. [abstract 22]. |
Complemento del título : |
Issue Section: Animal Breeding and Genetics. |
Fecha de publicación : |
2020 |
Fuente / Imprenta : |
Journal of Animal Science, 2020, Volume 98, Issue Supplement 4, Pages 6-7. doi: https://doi.org/10.1093/jas/skaa278.012 |
ISSN : |
1525-3163 |
DOI : |
10.1093/jas/skaa278.012 |
Idioma : |
Inglés |
Notas : |
Article history: 30 November 2020.
ASAS Annual 2020 Meeting Abstracts. |
Contenido : |
ABSTRACT - With an ever-increasing number of genotyped animals, there is a question of whether to include all genotypes into single-step GBLUP (ssGBLUP) evaluations or to include only genotyped animals with phenotypes and use indirect predictions (IP) for the remaining young genotyped animals. Under ssGBLUP, SNP effects can be backsolved from GEBV, and IP can be calculated as the sum of SNP effects weighted by the gene content. |
Palabras claves : |
Interim evaluations; SNP PEC. |
Asunto categoría : |
L10 Genética y mejoramiento animal |
Marc : |
LEADER 01308nam a2200241 a 4500 001 1063292 005 2022-06-14 008 2020 bl uuuu u01u1 u #d 022 $a1525-3163 024 7 $a10.1093/jas/skaa278.012$2DOI 100 1 $aGARCÍA, A. 245 $aAccuracy of indirect predictions for large datasets based on prediction error covariance of SNP effects from single-step GBLUP. [abstract 22].$h[electronic resource] 260 $aJournal of Animal Science, 2020, Volume 98, Issue Supplement 4, Pages 6-7. doi: https://doi.org/10.1093/jas/skaa278.012$c1093 500 $aArticle history: 30 November 2020. ASAS Annual 2020 Meeting Abstracts. 520 $aABSTRACT - With an ever-increasing number of genotyped animals, there is a question of whether to include all genotypes into single-step GBLUP (ssGBLUP) evaluations or to include only genotyped animals with phenotypes and use indirect predictions (IP) for the remaining young genotyped animals. Under ssGBLUP, SNP effects can be backsolved from GEBV, and IP can be calculated as the sum of SNP effects weighted by the gene content. 653 $aInterim evaluations 653 $aSNP PEC 700 1 $aAGUILAR, I. 700 1 $aLEGARRA, A. 700 1 $aMILLER, S. 700 1 $aTSURUTA, S. 700 1 $aMISZTAL, I. 700 1 $aLOURENCO, D.
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| Acceso al texto completo restringido a Biblioteca INIA Las Brujas. Por información adicional contacte bibliolb@inia.org.uy. |
Registro completo
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Biblioteca (s) : |
INIA Las Brujas. |
Fecha actual : |
24/01/2019 |
Actualizado : |
24/01/2019 |
Tipo de producción científica : |
Artículos en Revistas Indexadas Internacionales |
Circulación / Nivel : |
Internacional - -- |
Autor : |
FEDERICI, M.; BRANDA, A.; ARTIGAS, R.; DUTRA, F.; LLAMBÍ, S. |
Afiliación : |
MARIA TERESA FEDERICI RODRIGUEZ, INIA (Instituto Nacional de Investigación Agropecuaria), Uruguay; ANDREA BRANDA SICA, INIA (Instituto Nacional de Investigación Agropecuaria), Uruguay; RODY ARTIGAS, Universidad de la República (UdelaR)/ Facultad de Veterinaria; FERNANDO DUTRA, MGAP/ DILAVE (División de Laboratorios de Veterinarios); SILVIA LLAMBÍ, Universidad de la República (UdelaR)/ Facultad de Veterinaria. |
Título : |
High- resolution melting (HRM) curve analysis: New approach used to detect blad and dumps in Uruguayan Holstein breed. [Análise da curva de alta resolucao (HRM): Nova abordagem usada para detectar blad e dumps em bovinos da raça Holandesa Uruguaia]. |
Fecha de publicación : |
2018 |
Fuente / Imprenta : |
Archives of Veterinary Science, 2018, volume 23, Issue 4, pages 1-9. OPEN ACCESS. |
DOI : |
10.5380/avs.v23i4.62578 |
Idioma : |
Inglés |
Notas : |
Article history: Received 29 October 2018 // Accepted 29 November 2018.
This research was supported by the Unit of Biotechnology of INIA Las Brujas. We thank Dr. Marco Dalla Rizza, coordinator of the Unit of Biotechnology, as well as the staff of DNA Bank, located at the same Institute. |
Contenido : |
ABSTRACT.
The widespread use of artificial insemination has allowed the expansion of genetic progress. However, it also brought consequences such as the expansion of lethal hereditary diseases and the increase in inbreeding. The object of this study was to establish a fast and sensitive molecular assay to detect bovine leukocyte adhesion deficiency (BLAD) and deficiency of uridine monophosphate synthase (DUMPS) carriers in Uruguayan Holstein cattle by means of high resolution melting (HRM) curve analysis. By testing previously confirmed carrier and non-carrier animals, we set up a rapid, simple, and inexpensive diagnostic test using PCR followed by HRM curve analysis. The PCR-HRM genotyping method was effective for the discrimination of BLAD and DUMPS homozygous genotypes, and the BLAD heterozygous genotype. We conclude that the PCR-HRM assay is a robust, reliable, and economical tool for the detection of these mutations in the Holstein breed, which may be implemented in genetic selection programs.
© 2018, Universidade Federal do Parana.
RESUMO.
O uso generalizado de inseminação artificial, permitiu expandir o progresso genético, no entanto, também trouxe consequências na expansão de doenças hereditárias letais e aumento da endogamia. O objetivo deste estudo foi estabelecer um ensaio molecular rápido e sensível para detectar animais portadores da deficiência de adesão leucocitária bovina (BLAD) e da deficiência da uridina monofosfato sintetase (DUMPS) usando a análise de curva de alta resolução (HRM) em bovinos da raça Holandesa Uruguaia. Através do uso de animais portadores e não portadores previamente confirmados, montamos um teste de diagnóstico rápido, simples e de baixo custo usando PCR seguido de análise de curva HRM. O método de genotipagem da PCR-HRM foi efetivo para a discriminação dos genótipos homozigotos BLAD e DUMPS e do genótipo heterozigoto BLAD. Concluímos que o ensaio PCR-HRM é uma ferramenta poderosa, confiável e econômica para a detecção destas mutações na raça Holandesa, o que poderia ser implementado em programas de seleção genética.
© 2018, Universidade Federal do Parana. MenosABSTRACT.
The widespread use of artificial insemination has allowed the expansion of genetic progress. However, it also brought consequences such as the expansion of lethal hereditary diseases and the increase in inbreeding. The object of this study was to establish a fast and sensitive molecular assay to detect bovine leukocyte adhesion deficiency (BLAD) and deficiency of uridine monophosphate synthase (DUMPS) carriers in Uruguayan Holstein cattle by means of high resolution melting (HRM) curve analysis. By testing previously confirmed carrier and non-carrier animals, we set up a rapid, simple, and inexpensive diagnostic test using PCR followed by HRM curve analysis. The PCR-HRM genotyping method was effective for the discrimination of BLAD and DUMPS homozygous genotypes, and the BLAD heterozygous genotype. We conclude that the PCR-HRM assay is a robust, reliable, and economical tool for the detection of these mutations in the Holstein breed, which may be implemented in genetic selection programs.
© 2018, Universidade Federal do Parana.
RESUMO.
O uso generalizado de inseminação artificial, permitiu expandir o progresso genético, no entanto, também trouxe consequências na expansão de doenças hereditárias letais e aumento da endogamia. O objetivo deste estudo foi estabelecer um ensaio molecular rápido e sensível para detectar animais portadores da deficiência de adesão leucocitária bovina (BLAD) e da deficiência da uridina monofosfato sintetase (DUMPS) usando a análise de c... Presentar Todo |
Palabras claves : |
BLAD; DUMPS; HEIFER; HIGH-RESOLUTION DISSOCIATION CURVE; HOLSTEIN. |
Asunto categoría : |
-- |
Marc : |
LEADER 03353naa a2200253 a 4500 001 1059450 005 2019-01-24 008 2018 bl uuuu u00u1 u #d 024 7 $a10.5380/avs.v23i4.62578$2DOI 100 1 $aFEDERICI, M. 245 $aHigh- resolution melting (HRM) curve analysis$bNew approach used to detect blad and dumps in Uruguayan Holstein breed. [Análise da curva de alta resolucao (HRM): Nova abordagem usada para detectar blad e dumps em bovinos da raça Holandesa Uruguaia].$h[electronic resource] 260 $c2018 500 $aArticle history: Received 29 October 2018 // Accepted 29 November 2018. This research was supported by the Unit of Biotechnology of INIA Las Brujas. We thank Dr. Marco Dalla Rizza, coordinator of the Unit of Biotechnology, as well as the staff of DNA Bank, located at the same Institute. 520 $aABSTRACT. The widespread use of artificial insemination has allowed the expansion of genetic progress. However, it also brought consequences such as the expansion of lethal hereditary diseases and the increase in inbreeding. The object of this study was to establish a fast and sensitive molecular assay to detect bovine leukocyte adhesion deficiency (BLAD) and deficiency of uridine monophosphate synthase (DUMPS) carriers in Uruguayan Holstein cattle by means of high resolution melting (HRM) curve analysis. By testing previously confirmed carrier and non-carrier animals, we set up a rapid, simple, and inexpensive diagnostic test using PCR followed by HRM curve analysis. The PCR-HRM genotyping method was effective for the discrimination of BLAD and DUMPS homozygous genotypes, and the BLAD heterozygous genotype. We conclude that the PCR-HRM assay is a robust, reliable, and economical tool for the detection of these mutations in the Holstein breed, which may be implemented in genetic selection programs. © 2018, Universidade Federal do Parana. RESUMO. O uso generalizado de inseminação artificial, permitiu expandir o progresso genético, no entanto, também trouxe consequências na expansão de doenças hereditárias letais e aumento da endogamia. O objetivo deste estudo foi estabelecer um ensaio molecular rápido e sensível para detectar animais portadores da deficiência de adesão leucocitária bovina (BLAD) e da deficiência da uridina monofosfato sintetase (DUMPS) usando a análise de curva de alta resolução (HRM) em bovinos da raça Holandesa Uruguaia. Através do uso de animais portadores e não portadores previamente confirmados, montamos um teste de diagnóstico rápido, simples e de baixo custo usando PCR seguido de análise de curva HRM. O método de genotipagem da PCR-HRM foi efetivo para a discriminação dos genótipos homozigotos BLAD e DUMPS e do genótipo heterozigoto BLAD. Concluímos que o ensaio PCR-HRM é uma ferramenta poderosa, confiável e econômica para a detecção destas mutações na raça Holandesa, o que poderia ser implementado em programas de seleção genética. © 2018, Universidade Federal do Parana. 653 $aBLAD 653 $aDUMPS 653 $aHEIFER 653 $aHIGH-RESOLUTION DISSOCIATION CURVE 653 $aHOLSTEIN 700 1 $aBRANDA, A. 700 1 $aARTIGAS, R. 700 1 $aDUTRA, F. 700 1 $aLLAMBÍ, S. 773 $tArchives of Veterinary Science, 2018, volume 23, Issue 4, pages 1-9. OPEN ACCESS.
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